Semifinal
jeudi 27 juillet 2017, 17h41 GMT – 17h41 GMT
| Nom | Équipe | Temps | |
|---|---|---|---|
| 1 |  Sarah Sjöström | Sweden | 52.44″ |
| 2 |  Simone Manuel | United States | + 0.25″ |
| 3 |  Mallory Comerford | United States | + 0.41″ |
| 4 |  Pernille Blume | Denmark | + 0.55″ |
| 5 |  Bronte Campbell | Australia | + 0.6″ |
| 6 |  Penny Oleksiak | Canada | + 0.61″ |
| 7 |  Ranomi Kromowidjojo | Netherlands | + 0.65″ |
| 8 | Emma McKeon | Australia | + 0.76″ |
| 9 | Michelle Coleman | Sweden | + 1.07″ |
| 10 |  Charlotte Bonnet | France | + 1.33″ |
| 11 |  Menghui Zhu | China | + 1.41″ |
| 12 | Freya Anderson | Great Britain | + 1.47″ |
| 13 | Sandrine Mainville | Canada | + 1.57″ |
| 14 | Siobhán Haughey | Hong Kong, China | + 1.61″ |
| 15 |  Federica Pellegrini | Italy | + 1.82″ |
| 16 |  Andrea Murez | Israel | + 2.18″ |