Semifinal 2
vendredi 21 mai 2021, 16h58 GMT – 16h58 GMT
| Nom | Équipe | Temps | |
|---|---|---|---|
| 1 |  Adam Peaty | Great Britain | 26.38″ |
| 2 |  Ilya Shymanovich | Belarus | + 0.09″ |
| 3 | Lucas Matzerath | Germany | + 0.76″ |
| 4 | Kirill Prigoda | Russia | + 0.78″ |
| 5 | Johannes Skagius | Sweden | + 0.95″ |
| 6 | Caba Siladji | Serbia | + 0.96″ |
| 7 | James Wilby | Great Britain | + 0.98″ |
| 8 | Olivér Gál | Hungary | + 1.41″ |