Heat 2
lundi 17 mai 2021, 08h00 GMT – 08h00 GMT
| Nom | Équipe | Temps | |
|---|---|---|---|
| 1 |  Katinka Hosszú | Hungary | 4′ 37.42″ |
| 2 |  Viktória Mihályvári-Farkas | Hungary | + 0.65″ |
| 3 | Zsuzsanna Jakabos | Hungary | + 3.08″ |
| 4 |  Anja Crevar | Serbia | + 3.72″ |
| 5 | Catalina Corró | Spain | + 6.07″ |
| 6 |  Sara Franceschi | Italy | + 7.23″ |
| 7 | Katie Shanahan | Great Britain | + 9.86″ |
| 8 | Iman Avdic | Bosnia & Herzegovina | + 29.46″ |